Team:SJTU-BioX-Shanghai

This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled.

We aimed at constructing a set of protein assemblies on E.coli inner membrane as scaffold carrying various enzymes. Distinct from previous synthetic scaffold system, our device limits the reaction space to a two-dimensional surface. In such system, the membrane functions as an extensive scaffold for proteins to anchor without limitation of scaffold amount. Membrane as scaffold also has privilege in receiving external and internal regulating signals. Based on Membrane Scaffold, we built two universal devices: Membrane Accelerator and Membrane Rudder.

In Membrane Accelerator device, by gathering enzymes on membrane, production of fatty acid was enhanced by more than 24 fold through recruiting membrane accelerator system, which has a promising application prospect in biofuel production. We also proposed a new direction for application of scaffold-based accelerator: Biodegradation.

In Membrane Rudder device, we changed the direction of Violacein synthetic pathway through external signal.

ü The honors we won: Regional Winner, Best New BioBrick Part or Device Engineered and Gold Medal at Asia Jamboree; Advanced to World Championship.

ü The breakthrough we made: Redefinition of scaffold in Synthetic Biology by recruiting E.coli’s inner membrane as a natural two-dimensional scaffold.

ü The system we built: 6 membrane proteins orderly organized on the inner membrane of E.coli, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.

ü Device 1 we created – Membrane Accelerator: A universal tool that serves to accelerate biochemical reactions in E.coli; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type E.coli and 9 fold compared to E.coli overexpressing cytoplasmic enzymes.

ü Device 2 we created – Membrane Rudder: A universal tool used to dynamically and artificially control biochemical reactions in E.coli; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched.

ü New direction we proposed: The application of scaffold system in accelerating biodegradation pathway using our Membrane Accelerator.

ü Parts we submitted: 42 well-characterized parts that could either be used directly or serve as a universal tool readily for potential scientific or engineering use.

ü A club we established – BioCraft: The headquarter of our human practice programs, having come a long way in propagandizing Synthetic Biology and iGEM. Warmly-received activities have been held in and outside the campus. Several celebrities in different fields have shown support for us, laying a cornerstone for our future development.