Team:Potsdam Bioware/Lab/Labjournal/October

AID

2012-10-10

Inoculation of plasmid samples of the 48h retransformation plates (after FACS)

Investigators: Tom

Time: 2012-10-10

Materials:

• LB medium
  

• Amp stock solution
  

• plate with cultures:
  

EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP

(after FACS selection)

Method:

Inoculation of:

5 cultures per plate in 5 ml LB medium + 5µL Amp.

(--> 5 cultures)

Further tasks:

• Miniprep
  

Antibody

2012-10-12

cell culture

Investigator:Kerstin

Topic: cell-culture

• passaging of HT1080
• dilution of stably transfected cells (Nanobody construct clone 29, originally from transfected Well 3) to 1-2 cells per 150µl and seeding in 4x 96-Well plates
• seeding of stably transfected CHO Clone 29 cells in Ibidi Dish
• change of medium in 75cm² flasks with stably transfected cells (Clone 4 and 29)
• harvest cells for Western Blot (CHO not transfected, CHO stably transfected Clone 4 and 29)

Virus

XXX

XXX

Investigator: XXX

Aim: XXX

Materials:

XXX

Method:

XXX

Results:

XXX

Further tasks