Team:Potsdam Bioware/Lab/Labjournal/October

AID

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Investigators:

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Aim:

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Method:

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Results:

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Antibody

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Investigator:XXX

Time: XXX

Materials: XXX

Methods: XXX

Results:

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Further tasks: XXX

Virus

2012-09-02

Topic: preparing viral stocks

Investigator: Kathi

Aim: preparation of the primary virus stock

Materials:

• cell-culture --> HEK AAV 293 transfected cells on 29.08.2012 (with following plasmids: p01, p06, phelper, psb1c3)

• 80 °C freezer

• 37 °C water bath
  

Method:

• transfer transfected cells (incl. DMEM) to a falcon tube

• 4 times: 10 min freeze the falcon tube (-80 °C) and 15 min unfreezing (37 °C water bath)

• centrifugation (10 min, 10000 g, RT)

• supernatant = virus stock

• sore at -80 °C
  

Results:

• virus stock → with YFP on the surface and GOI = CFP
  

Further tasks

• infection