Team:Potsdam Bioware/Lab/Labjournal/October

AID

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XXX

Investigators:

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Aim:

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Method:

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Results:

XXX

Antibody

2012-09-01

Glycerol stock and Mini Prep of Geneart construct, Venus, pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2

Investigator:Maria

Time: 2012-09-01 9:30 am

Materials: overnight cultures Geneart construct (4x), Venus (Tom), pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2 (Chris), Mini prep kit

Methods: according to manual

Results:

Venus: …. ng/µl

pAK100+scFv425 R H1: --- ng/µl

pAK100+scFv425 R H2: --- ng/µl<br<

pAK100+scFv425 R L1: --- ng/µl

pAK100+scFv425 R L2: --- ng/µl

pAK100+scFv425 L1: --- ng/µl

pAK100+scFv425 L2: --- ng/µl

Geneart construct 1: --- ng/µl

Geneart construct 2: --- ng/µl

Geneart construct 3: --- ng/µl

Geneart construct 4: --- ng/µl

+

Glycerole stocks of Geneart construct and pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2

Further tasks:

Virus

2012-09-02

Topic: preparing viral stocks

Investigator: Kathi

Aim: preparation of the primary virus stock

Materials:

• cell-culture --> HEK AAV 293 transfected cells on 29.08.2012 (with following plasmids: p01, p06, phelper, psb1c3)

• 80 °C freezer

• 37 °C water bath
  

Method:

• transfer transfected cells (incl. DMEM) to a falcon tube

• 4 times: 10 min freeze the falcon tube (-80 °C) and 15 min unfreezing (37 °C water bath)

• centrifugation (10 min, 10000 g, RT)

• supernatant = virus stock

• sore at -80 °C
  

Results:

• virus stock → with YFP on the surface and GOI = CFP
  

Further tasks

• infection