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Our Favorite BioBricks

BBa_K779500: Reporter top strand

Registry entry
One half of our reporter RNA complex. When this RNA strand is displaced from its bottom strand, the RQ fluorescence quencher on this strand separates from a ROX fluorescent molecule on the bottom strand, causing the bottom strand to fluoresce. We arrived at this design after numerous iterations over the course of the summer.

Characterization on Foundational page.

BBa_K779607: Tre-Tight-mKate inducible fluorescence with DOX

BBa_K779400: U6-TetO-FF1 inducible RNA expression

All BioBricks

Name	Type	Description	Designer	Length
BBa_K779100	DNA	Fuel 1 - mRNA domain 1 DNA sensor	Divya Israni	18
BBa_K779101	DNA	Gate 1 - mRNA domain 1 DNA sensor	Divya Israni	25
BBa_K779102	DNA	Output 1 - mRNA domain 1 DNA sensor	Divya Israni	33
BBa_K779103	DNA	Input Mimic 1 - mRNA domain 1 DNA sensor	Divya Israni	20
BBa_K779104	DNA	Fuel 2 - mRNA domain 2 DNA sensor	Divya Israni	18
BBa_K779105	DNA	Gate 2 - mRNA domain 2 DNA sensor	Divya Israni	25
BBa_K779106	DNA	Output 2 - mRNA domain 2 DNA sensor	Divya Israni	33
BBa_K779107	DNA	Input Mimic 2 - mRNA domain 2 DNA sensor	Divya Israni	20
BBa_K779108	DNA	Fuel 3 - mRNA domain 3 DNA sensor	Divya Israni	18
BBa_K779109	DNA	Gate 3 - mRNA domain 3 DNA sensor	Divya Israni	25
BBa_K779110	DNA	Output 3 - mRNA domain 3 DNA sensor	Divya Israni	33
BBa_K779111	DNA	Input Mimic 3 - mRNA domain 3 DNA sensor	Divya Israni	20
BBa_K779112	DNA	Fuel 4 - mRNA domain 4 DNA sensor	Divya Israni	18
BBa_K779113	DNA	Gate 4 - mRNA domain 4 DNA sensor	Divya Israni	33
BBa_K779114	DNA	Output 4 - mRNA domain 4 DNA sensor	Divya Israni	25
BBa_K779115	DNA	Input Mimic 4 - mRNA domain 4 DNA sensor	Divya Israni	20
BBa_K779116	RNA	Short RNA reporter bottom strand (with ROX fluorophore)	Divya Israni	20
BBa_K779117	RNA	RNA Reporter top strand with quencher (RQ)	Divya Israni	15
BBa_K779118	RNA	RNA Input S6	Divya Israni	20
BBa_K779119	RNA	RNA Control input (scrambled)	Divya Israni	20
BBa_K779120	RNA	RNA Reporter top strand with quencher (RQ) and tag fluorophore (Alexa 488)	Divya Israni	15
BBa_K779121	DNA	Short DNA reporter top strand (with RQ quencher)	Divya Israni	15
BBa_K779122	DNA	Short DNA reporter bottom strand (with ROX fluorophore)	Divya Israni	20
BBa_K779123	DNA	a - In vitro NOT gate	Divya Israni	43
BBa_K779124	DNA	b1 - In vitro NOT gate	Divya Israni	28
BBa_K779125	DNA	b2 - In vitro NOT gate	Divya Israni	20
BBa_K779126	DNA	c1 - In vitro NOT gate	Divya Israni	43
BBa_K779127	DNA	c2 - In vitro NOT gate	Divya Israni	29
BBa_K779128	DNA	c3 - In vitro NOT gate	Divya Israni	47
BBa_K779129	DNA	d1 - In vitro NOT gate	Divya Israni	29
BBa_K779130	DNA	d2 - In vitro NOT gate	Divya Israni	38
BBa_K779131	DNA	e - In vitro NOT gate	Divya Israni	34
BBa_K779200	Regulatory	Hef1A	Giulio Alighieri	1174
BBa_K779201	Regulatory	CMV	Giulio Alighieri	70
BBa_K779202	Regulatory	TRE-Tight	Giulio Alighieri	196
BBa_K779300	Reporter	eBFP2	Giulio Alighieri	726
BBa_K779301	Reporter	eYFP	Giulio Alighieri	726
BBa_K779302	Reporter	eYFP-4xFF1	Giulio Alighieri	859
BBa_K779303	Reporter	eYFP-4xFF4	Giulio Alighieri	835
BBa_K779304	Reporter	mKate	Giulio Alighieri	721
BBa_K779305	Coding	rtTA3	Giulio Alighieri	714
BBa_K779306	Reporter	TagBFP	Katie Bodner	699
BBa_K779307	Reporter	TagBFP-FF5	Katie Bodner	801
BBa_K779308	Coding	TetR	Katie Bodner	630
BBa_K779309	Reporter	eBFP2-1x	Katie Bodner	754
BBa_K779310	Reporter	eBFP2-2x	Katie Bodner	778
BBa_K779311	Reporter	HH-mKate - Hammerhead attached to front of mKate	Katie Bodner	802
BBa_K779312	Reporter	mKate-HH - Hammerhead attached behind mKate	Katie Bodner	804
BBa_K779313	Reporter	HHM-mKate - Repressible hammerhead attached to front of mKate	Felix Sun	838
BBa_K779314	Reporter	mKate-HHm - Repressible hammerhead behind mKate	Felix Sun	844
BBa_K779315	Coding	5' Hammerhead with loss-of-function mutation	Felix Sun	190
BBa_K779316	Coding	3' Hammerhead with loss-of-function mutation	Felix Sun	186
BBa_K779317	Coding	5' Hammerhead	Felix Sun	190
BBa_K779318	Coding	3' Hammerhead	Felix Sun	186
BBa_K779400	Regulatory	U6-TetO-FF1	Divya Israni	326
BBa_K779401	Regulatory	U6-TetO-S6	Divya Israni	277
BBa_K779402	Regulatory	U6-TetO-S1	Divya Israni	277
BBa_K779403	Regulatory	U6-TetO-FF4	Divya Israni	596
BBa_K779404	Regulatory	U6-TetO-DecoyFF4-3p	Divya Israni	287
BBa_K779405	Regulatory	U6-TetO-DecoyFF4-3p-4ntin	Divya Israni	291
BBa_K779406	Regulatory	U6-TetO-TuDFF4-3p	Divya Israni	390
BBa_K779407	Regulatory	U6-TetO-TuDFF4-3p-4ntin	Divya Israni	398
BBa_K779408	Regulatory	U6-TetO-Sa	Divya Israni	285
BBa_K779409	Regulatory	U6-TetO-Sc	Divya Israni	285
BBa_K779500	Other	Long reporter top strand with bulge	Kristjan Eerik Kaseniit	24
BBa_K779501	Other	Long reporter top strand	Kristjan Eerik Kaseniit	20
BBa_K779502	Other	Long reporter bottom strand	Kristjan Eerik Kaseniit	28
BBa_K779503	Other	Long input strand	Kristjan Eerik Kaseniit	28
BBa_K779504	Other	Long scrambled input strand	Kristjan Eerik Kaseniit	28
BBa_K779600	Generator	Hef1a-eYFP	Giulio Alighieri	1922
BBa_K779601	Generator	Hef1a-mKate	Giulio Alighieri	1917
BBa_K779602	Generator	Hef1a-TagBFP-FF5	Giulio Alighieri	1997
BBa_K779603	Generator	Hef1a-eYFP-4xFF1	Giulio Alighieri	1922
BBa_K779604	Generator	Hef1a-rTTA3	Giulio Alighieri	1926
BBa_K779605	Generator	Hef1A-TetR	Katie Bodner	1842
BBa_K779606	Generator	Tre-T-eYFP	Katie Bodner	944
BBa_K779607	Generator	Tre-Tight-mKate	Katie Bodner	939

Team:MIT/ResultsBiobricks