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Contents 1 Hello 2 March 2.1 Spring Boot Camp 2.1.1 Monday, March 5 2.1.2 Tuesday, March 6 2.1.3 Wednesday, March 7 2.1.4 Thursday, March 8 2.1.5 Friday, March 9 3 July 3.1 Ag43&Lysis team experiment 3.1.1 weak 1

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<)　
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

March

Spring Boot Camp

date

March 5 (Mon) ~ March 9 (Fri)

Monday, March 5

Session #1

Short lecture about moleculer biology (Mr.Yamazaki, our adviser)

Session #2

Tutrial: How to use 'Unipro UGENE' (iTakeshi)

Session #3

Guidance: Wiki Reading (Laury)

Example: 2010 MIT

Tuesday, March 6

Session #4~6

Reading Wikis in turn and discussions

• 2010 NYU
• 2009 Cambridge
• 2009 Growningen

Wednesday, March 7

Session #7~11

Reading Wikis (2)

• 2010 Washington
• 2009 Valencia
• 2011 Barklay
• 2010 Paris
• 2010 Bristol

Thursday, March 8

Session #12

2012 Project Brainstorming

The details is secret! :)

Session #13

Guidance: How to read papers (Laury)

Friday, March 9

Session #14

2012 Project Brainstorming (2)

Session #15

Guidance: How to look up papers you want (Laury)

Session #16

Tutorial: Modeling the behavior of cells (iTakeshi)

Session #17

Final Session: Reviewing this camp

Party!!

July

Ag43&Lysis team experiment

weak 1

• 4th

Transformation

1. Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43) in DH5α
2. Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated

• 5th

Transformation

K346007(Ag43) was failed to cultivate on LBC plate.

1. Transformation of K346007(Ag43) in DH5α.
2. Cultivated on LBC in 21hrs.

Single colony isolation

1. Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
2. Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins

BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

• 6th

Liquid culture

1. Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
2. LB　1ml is for glycerol stocks and 2ml is for mini-prep.
3. 16hrs Cultivation

Single colony isolation

1. Single colony isolation of K346007(Ag43).

• 7th

mini-prep

1. mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43.

Glycerol stock