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From 2012.igem.org

Parts Registry

NameDescriptionRegistry linkPart typeAvailability precA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).

BBa_K862000

Measurement parts registry psulA_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E.coli (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004). BBa_K862001 Measurement parts registry precB_LacZ

This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (BBa_K173004).BBa_K862002 Measurement parts registry precB

The RecB promoter sequence was obtained from the E.coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.

RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA
CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3'

RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC
GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'BBa_K862003 Regulatory can be cloned on oligo basis (sequence provided)