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From 2012.igem.org

Contents 1 Notebook for week of June 25 to July 2 1.1 June 25 1.1.1 Design of converter plasmid 1.1.2 Design of Reporter Plasmid 1.1.3 Spinach Aptamer 1.1.4 CBB5 Caffeine Inducible Promoters 1.1.5 Inducible Odor

Notebook for week of June 25 to July 2

June 25

Design of converter plasmid

• Razan
  • 6/26 - Tuesday: First assembly PCR for converter and control plasmid.
  • 6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

• Peter
  • 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
  • 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
  • 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.

Inducible Odor