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Kanomycin Plate Preparation
- Autoclave two 500mL LB-Agar solutions
- Add 0.5mL of stock kanomycin (50mg/mL) in one 500mL LB-Agar solution (final conc. 50ug/mL)
- Add 0.2mL of stock kanomycin (50mg/mL) in other 500mL LB-Agar solution (final conc. 20ug/mL)
- Pour our solutions into 20 plates each
- Wait for them to cool, then flip and bag
- Store in deli fridge
Lab Notes, August, 6, 2012
Electroporation Preparation - Buffers
- Prepare electroporation and washing buffers for B.Subtilis
- Washing Buffer
- 0.25M Sucrose
- 1mM Hepes
- MgCl2
- (pH - 7.0)
- 0.3M Sucrose
- 1mM CaCl2
- 1mM Sodium citrate
- (pH - 6.0)
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