User:Jn323/30 July 2012

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Preparation for Electroporation

  • Inoculate cells into 10mL of LB media (scrape glycerol stock with metal loop and place into LB solution in culture tube)
  • ADP1, EcNR2, BS168, BS PERM739, BS PY79, BS 1A833, XL1-Blue
  • Place culture tubes in shaking incubator
  • Transfer solution to 125mL E.flasks and add 15mL of LB media
  • Inoculate cells onto appropriate LB plates for overnight growth
  • same cells minus XL1-Blue
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