From 2012.igem.org
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I. Ordered Cross-over PCR Primers from Keck
- Primers were ordered with the name/sequence in the following table:
| Name | Tm (Tm overhang/Tm anneal) | Length (bp) | Sequence (overhangANNEAL)
|
| cT5_p1f | 58.79 | 20 | 5'-TTTGCTTTGTGAGCGGATAA-3'
|
| cT5_p1r | 72.34 (63.39/57.26) | 50 (25/25) | 5'-ccagtgaatccgtaatcatggtcatCTAGTATTTCTCCTCTTTCTCTAGT-3'
|
| cT5_p2f | 70.69 (61.21/55.65) | 52 (32/20) | 5'-aacaattactagagaaagaggagaaatactagATGACCATGATTACGGATTC-3'
|
| cT5_p2r | 71.82 (62.57/55.41) | 50 (30/20) | 5'-tctccatacctcatttctcctctttattacTTATTTTTGACACCAGACCA-3'
|
| cT5_p3f | 70.56 (61.17/56.56) | 54 (32/22) | 5'-caaaaataagtaataaagaggagaaatgaggtATGGAGAAAAAAATCACTGGAT-3'
|
| cT5_p3r | 72.61 (62.18/58.07) | 50 (37/13) | 5'-ttgttagatttcatatattctttctcctctttcttaaTTACGCCCCGCCC-3'
|
| cT5_p4f | 71.04 (62.15/56.45) | 50 (30/20) | 5'-ggcgtaattaagaaagaggagaaagaatatATGAAATCTAACAATGCGCT-3'
|
| cT5_p4r | 75.27 (63.03/57.88) | 40 (25/15) | 5'-gagcctttcgttttatttgatgcctTCAGGTCGAGGTGGC-3'
|
| cT5_p5f | 74.93 (61.12/55.71) | 37 (16/21) | 5'-ggccacctcgacctgaAGGCATCAAATAAAACGAAAG-3'
|
| cT5_p5r | 55.61 | 15 | 5'-GAGAGCGTTCACCGA-3'
|
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