"

From 2012.igem.org

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Contents 1 Hello 2 July 2.1 4th 2.2 5th 2.3 6th

Hello

We are team HokkaidoU Japan! Today we learn and start to edit wiki. (>ω<)　
Dear Mr.Ortiz, I saw the help page which you edited.
hola!

July

4th

Transformation

• Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
  • Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43)

5th

Transformation

• Transformation of K346007(Ag43)in DH5α.
  • Cultivation on LBC.

Single colony isolation

• Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
  • BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.

6th

Liquid culture

• Liquid culture in LBA(B0015,B0034) and LBC(K542009)
  • LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep

Single colony isolation

• Single colony isolation of K346007(Ag43).