Team:Washington/Protocols

From 2012.igem.org

Revision as of 23:42, 29 September 2012 by Nbolten (Talk | contribs)


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagenesis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Gibson Cloning/Assembly

Isolation of Plasmid DNA (miniprep)

Preparation of Overnight Cultures

Plastic Degradation

[Protocols]

Optogenetics

Light App Experiments

Flu Binders

[Protocols]