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From 2012.igem.org

Team:Michigan/Notebook/Protocols

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Modify HbiF into a Biobrick compatible format

HbiF and LVA degradation tag were ordered from IDT with the following specifications.

EcoRI

XbaI

SpeI

PstI

AgeI

NgoMIV

HbiF possesses an N-terminus in the (truncated) RFC10 format :

GAATTC GCGGCCGC T TCTAG [ATG]

Prepare FimE for protein expression

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