Team:Amsterdam/project/biobricks

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BioBricks

<groupparts>iGEM12 Amsterdam</groupparts>

Contents

Favourite Constructs

IPTG inducible expression of M.ScaI methyltransferase
(IPTG -> M.ScaI) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K874100 BBa_K874100]

This BioBrick contains the first proof of concept and contains the both the Reader and the Sensor described in our molecular design. Therefore this is also the most extensively studied BioBrick in our project, for an in dept view of the experiments we performed using this BioBrick you can look at the Experimental Setup section. We managed to insert this BioBrick in both the pSB1AT3 and pSB1C3 backbones but all testing was done in pSB1AT3.