Team:SDU-Denmark/Project/BioBricks
From 2012.igem.org
BioBricks
See our submitted BioBricks and composite parts
Primary nucleaic acid sequence
The gene sequence can be found through the link by clicking on 'Get selected sequences'.
The same goes for the FFT. Here is the link
Description of function
Sucrose:sucrose fructosyltransferase (SST) is one out of two necessary enzymes to produce inulin. The function of SST is to make a tri-saccharide with sucrose as substrate. Two molecules of sucrose is transformed to either 1-kestose or neokestose which is a precursor to inulin. Fructan:Fructan fructosyltransferase (FFT) is the next necessary enzyme in inulin production. This enzyme has the ability to polymerize the tri-saccharide SST made and elongate the chain with fructose molecules. Both enzymes is from the glycosyltransferase family and are originally expressed in some plants.
Authorship
The original idea for the project is built entirely on the thoughts and ideas of the team's ten participants. All theoretical text and hypotheses about the project is likewise done by the iGEM team. We have put extra focus on marketing our final product and in the context teamed up with two with a degree in international business and law, which has helped us 'Business Aspects'. See Attributions for a detailed description of them.
Safety notes
See our kill-switch system and considerations on avoiding horizontal gene transfer under Constructs