• Home
• The Team
  • Students
  • Advisors / Instructors
  • Attribution
  • Gallery
• GEMOTE Project
  • Abstract
  • Project description
  • Characterisation
  • Applications / Perspectives
  • Materials / Techniques
  • Notebook
    • Week 1
    • Week 2
    • Week 3
    • Week 4
    • Week 5
    • Week 6
    • Week 7
    • Week 8
    • Week 9
    • Week 10
    • Week 11
    • Week 12
    • Week 13
• Communication
  • Exposition
  • Scientific Meetings
  • Media
  • Future Projects
• Our Partners
  • Hosting Institution
  • Sponsors
• Safety
• Contact Us

Week 10

6th August

• Sending of the B1 sample to sequencing with two pairs of primers
  • K-274100 Forward and Reverse
  • Plasmid pSB1A2 Forward and Reverse

7th August

• Liquid culture of B1 in order to prepare a glycerol stock
  • Receipt of new primers
  • 2 Forward
  • 3 Reverse
  • Plasmid Reverse

8th August

Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.

PCR program used:

9th August

New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.

• Digestion by EcoRI of the plasmid that contains BBa_K274100.

10th August

Amplification by PCR of BBa_K274100 already digested by EcoRI. Visualization by electrophoresis on a 0.8% Agarose gel.

PCR program used:

Miniprep of BBa_K274100 followed by Nanovue to determine the concentration of the sample
Miniprep of the Plasmid pSB1A2 followed by Nanovue to determine the concentration of the sample

• Digestion by HindIII of the plasmid pSB1A2 in order to linearize it.

Digestion of BBa_K115017 by DPNI to eliminate the plasmid matrix. Visualization by electrophoresis on a 2% Agarose gel. We are expecting a band at 123 bp.