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Week 4

• We placed an order for the primers.
• DNA (biobricks) Resuspension using iGEM protocol.
• Thermal shock treatment of each biobrick to have a large quantity of DNA.
• Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
• We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
• Miniprep to purify each biobrick (ready to use).
• Groove for two colonies because they did not grow on the liquid culture.