From 2012.igem.org
Weeks:
| June |
July |
August |
September |
October |
November |
Week 14: 10th September - 16th September
this week we have worked extensively on a batch of different manipulations including:
- biobricking promotors in Psb1c3 which are:
- P Elastase
- Hsp70
- P Car A
- FLk1
- P CMV
this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:
this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles
- Preparation of chemical Competent Cells
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