Team:Exeter/lab book/1gp/wk2

Operon Construction  |  Glycobase

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• BioBrick extraction of RBS BioBrick (BBa_B0034) and TetR repressible promoter (BBa_R0040)

• Transformation of RBS BioBrick and TetR promoter

Tuesday 17th July (15.00)

• Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight

Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic.

Wednesday 18th July (15.00)

• Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight

No cultures were found in all liquid mediums and thus repeated.

Thursday 18th July (9.00)

• Mini-Prepping of RBS and TetR promoter

• Gel Electrophoresis to check fragment sizes of RBS and TetR promoter

Gel Electrophoresis showed that RBS and TetR promoter were successfully cloned. Lane 1 = DNA hyperladder, Lane 3 = TetR promoter mini-prep 2 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 4 = TetR promoter mini-prep 3 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 5 = TetR promoter mini-prep 4 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 6 = RBS mini-prep 1 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 7 = RBS mini-prep 2 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 8 = RBS mini-prep 3 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 9 = RBS mini-prep 4 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 10 = DNA hyperladder.