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Team:University College London/Protocols/Week13/2
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Revision as of 22:35, 26 September 2012 by Erinoerton (Talk | contribs)
Ligations for irrE, nuclease, laccase & curli
Digest upstream part with EcoRI-HF™ and SpeI
| Constitutive promoter-rbs construct | 500 ng |
|---|---|
| EcoRI-HF | 1µl |
| Spel | 1 µl |
| 10X NEBuffer 2 | 5 µl |
| 100X BSA | 0.5 µl |
| H2O | to 50 µl |
Digest downstream part with Xbal and Pstl
| Curli (from PCR product) Laccase (from PCR product) irrE (from PCR product) Nuclease (in puc57) | 500 ng |
|---|---|
| Xbal | 1 µl |
| Pstl | 1 µl |
| 10X NEBuffer 2 | 5 µl |
| 100X BSA | 0.5 µl |
| H2O | to 50 µl |
