Team:UT-Tokyo/LabWork/RegularMethods

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Regular Methods

Assembly parts

Digest

Materials

Protocol

Ligation

Materials

  • Vector DNA
  • Insert DNA
  • 2x Ligation Mix

Protocol

1.Make reaction liquid
  • MilliQ up to 20uL
  • 10uL 2x Ligation Mix
  • Vector DNA
  • Insert DNA
2.Incubation at 16 °C for 15-30 min.

Transformation

Purification of DNA

Reagents

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