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1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (P_veg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms.

2. Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.

3. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (P_veg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.
Main Page: P_lac(B. subtilis)-RBS(E. coli)-GFP BBa_K802002 : This part is used to determine if the P_lac promoter works or not and especially how it works when it is activated by different concentration of the inducer IPTG. Within this part there is P_lac from Bacillus subtilis which is the promoter that induces production of XylR to form a positive biofilm. Then a RBS from E coli is added to finally enable production of GFP which is an easy measurable parameter using the amount of fluorescence.


Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. We determined antibiotic resistance thresholds and transformation efficiencies and compared them to BBa_K802003

Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production (an antimicrobial lipopeptide) and to repress the biofilm formation in B. subtilis strains.