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Team:UT-Tokyo/LabWork/RegularMethods
From 2012.igem.org
Regular Methods
Assembly parts
Digest
Materials
Protocol
Ligation
Materials
- Vector DNA
- Insert DNA
- 2x Ligation Mix
Protocol
1.Make reaction liquid --MilliQ up to 20uL --10uL 2x Ligation Mix --Vector DNA --Insert DNA 2.Incubation at 16 °C for 15-30 min.
