Team:Exeter/lab book/1gp/wk2

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ExiGEM2012 Lab Book 1GP wk2


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 17th - 21st September - 24th - 28th September - Results
	Single Gene Plasmids and Enzyme Characterisation: 16th - 20th July 2012 Monday 16th July (11.00) • BioBrick extraction of RBS BioBrick (BBa_B0034) and TetR repressible promoter (BBa_R0040) • Transformation of RBS BioBrick and TetR promoter Tuesday 17th July (15.00) • Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic. Wednesday 18th July (15.00) • Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight No cultures were found in all liquid mediums and thus repeated. Thursday 18th July (9.00) • Mini-Prepping of RBS and TetR promoter • Gel Electrophoresis to check fragment sizes of RBS and TetR promoter Gel Electrophoresis showed that RBS and TetR promoter were successfully cloned. Lane 1 = DNA hyperladder, Lane 3 = TetR promoter mini-prep 2 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 4 = TetR promoter mini-prep 3 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 5 = TetR promoter mini-prep 4 (expected: 2155bp since TetR promoter is too small to be seen at 54bp), Lane 6 = RBS mini-prep 1 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 7 = RBS mini-prep 2 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 8 = RBS mini-prep 3 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 9 = RBS mini-prep 4 (expected: 2155bp since RBS is too small to be seen at 12bp), Lane 10 = DNA hyperladder.