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Team:ZJU-China/n p 7.htm
From 2012.igem.org
7 PCR:
7.1 Use TaKaRa Premix Taq Version 2.0. Follow the manufacturer’s protocols.
7.2 Set up the following reaction in a PCR tube on ice.
| Premix Taq | 12.5 μl |
| Template | 0.1 ng - 10 ng |
| Primer F | 0.5 μl |
| Primer R | 0.5 μl |
| Nuclease-free water | To 25 μl |
| Total volume | 25 μl |
7.3 PCR cycle:
94℃ 3min
30cycles
94℃ 30s
55℃ 30s
72℃ 1min
72℃ 5min
