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From 2012.igem.org

1 Stock solutions

 

1.1 Ampicillin: 100 mg/mL.

1 g Amp + 10mL water. Filter sterilize, freeze in aliquots.

 

1.2 Kanamycin: 50 mg/mL

0.5 g Kan + 10mL water. Filter sterilize, freeze in aliquots.

 

1.3 Spectinomycin: 10 mg/mL

0.1 g Amp + 10mL water. Filter sterilize, freeze in aliquots.

 

1.4 Chloramphenicol:

 

1.5 1M IPTG:

2.4 grams +10mL Water. Filter sterilize, freeze in aliquots.

 

1.6 50×TAE electrophoresis buffer:

Tris base 242 g

Glacial acetic acid 57.1ml

Na2EDTA•2H2O 37.2 g

H2O to 1 liter

 

1.7 LB medium

Tryptone 10 g

Yeast Extract 5 g

NaCl 10 g

PH？

Add H2O to 1L