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Week 4

• We place an order for the primers
• DNA (biobricks) Resuspension using iGEM protocol
• Transformation of each biobricks by thermal shock to have a large quantity of DNA
• Staggering of the transformed bacteria on a selective medium to select bacteria which received the plasmid
• We put some colonies, for each biobricks, on liquid culture to be able to make a Miniprep
• Miniprep to purify each biobrick (ready to use)
• Groove for two colonies because they did not grown on the liquid culture