Team:University College London/Protocols/T4dnaligase

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Transformation Protocol 2

Nuclease positive cells

COMPONENT	20 μl REACTION)
10X T4 DNA Ligase Buffer	2 μl
Vector DNA (3 kb)	50 ng (0.025 pmol)
Insert DNA (1 kb	50 ng (0.076 pmol)
Nuclease-free water	to 20 μl
T4 DNA Ligase	1 μl

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