Team:XMU-China/protocolsindex

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  • 1 General protocols
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  •        1.1 Stock solution
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  •        1.2 Preparation of Competent BL21
  •        1.3 Transformation
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  •        1.4 Plasmid Purification
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  •        1.5 Reaction system of restriction endonuclease
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  •        1.6 Standard BioBrick Assembly
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  •        1.7 Suffix Insertion
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  •        1.8 Ligation
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  •        1.9 Restriction analysis
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  •        1.10 Gel Extraction
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  • 2 Characterization
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  •        2.1 Fluorescence Measurements
  •        2.2 Protein electrophoresis
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  •               2.2.1 Preparation of Samples
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  •               2.2.2 Manufacture Albumen Gel
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  •               2.2.3 Electrophoresis
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  •               2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
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  •               2.2.5 Scanning
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  • 3 Immobilization
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  •        3.1 Prepare Sodiumcellu-losesulfate (NaCS)
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  •        3.2 Prepare microcapsules
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  •        3.3 Immobilize cells into calcium alginate beads
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  •        3.4 Immobilize cells into intra-hollow Ca-alginate capsules
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  •        3.5 Immobilize cells into NaCS-PDMDAAC microcapsules
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