Team:Evry-Genopole/Notebook/June/29

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Team IGEM Evry 2012

Promoters & Reporters workgroup

Gel migration
Gel preparation
25mL agarose+TAE (frigo) + 2microL BET
Sample preparation
DNA Ladder 1kB: 1microL ladder + 1microL loading buffer + 4microL ddH2O
Digested RFP, YFP, CFP, GFP, pCS2+ (from 28 june) : 10microL sample + 2microL loading buffer
=> 100V, 1h