Team:University College London/LabBook/Week15
From 2012.igem.org
15.1
Moday (17.09.12) 3A assembly
Aim – Start 3A assembly to ligate:
- the first (BBa_K729004 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct
- the first (BBa_K729003 and plasmid backbone PSB1C3 ) and second ( BBa_J23119 +BBa_0034) construct
- the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct
- the first (plasmid backbone PSB1K3 and BBa_K118011 ) and second ( BBA_I746916 and plasmid backbone PSB1K3) construct
- pstA promoter (BBA_I719005) and the GFP (BBA_I712069)
- the Biobricks BBa_I746909 and BBa_K729007 to generate a Biobrick that contains pcstA-T7RNAP-pT7-GFP-TT. </p>
15.2