Team:Paris-Saclay/Project/Notebook/Week 7

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  • PCR in large volume to have a lot a available biobricks
  • Treatment with DPN1 to eliminate plasmids
  • Gel purification to only keep our interest fragments
  • Gibson Assembly method to put together the different biobricks for each construction
  • Transformation with assembly products and staggering on a selective medium
  • Colony PCR to have results before the transformation results