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Team:TU Darmstadt/Protocols/GC-MS/GC-Protocol
From 2012.igem.org
Contents |
INSTRUMENT CONTROL PARAMETERS
Control Information
- Sample Inlet : GC
- Injection Source : GC ALS
- Mass Spectrometer : Enabled
Oven
- Equilibration Time 0.25 min
- Oven Program On
55 °C for 0.5 min then 30 °C/min to 250 °C for 3 min
- Run Time 10 min
Front Injector
- Syringe Size 10 µL
- Injection Volume 1 µL
- Injection Repetitions 1
- Solvent A Washes (PreInj) 2
- Solvent A Washes (PostInj) 2
- Solvent A Volume 5 µL
- Solvent B Washes (PreInj) 2
- Solvent B Washes (PostInj) 2
- Solvent B Volume 5 µL
- Sample Washes 1
- Sample Wash Volume 1 µL
- Sample Pumps 2
- Dwell Time (PreInj) 0 min
- Dwell Time (PostInj) 0 min
- Solvent Wash Draw Speed 300 µL/min
- Solvent Wash Dispense Speed 6000 µL/min
- Sample Wash Draw Speed 300 µL/min
- Sample Wash Dispense Speed 6000 µL/min
- Injection Dispense Speed 6000 µL/min
- Viscosity Delay 1 sec
- Sample Depth Disabled
Back Injector
- Front SS Inlet He
- Mode Pulsed Splitless
- Heater On 270 °C
- Pressure On 0.54761 bar
- Total Flow On 54 mL/min
- Septum Purge Flow On 3 mL/min
- Gas Saver On 15 mL/min After 3 min
- Injection Pulse Pressure 1 bar Until 0.5 min
- Purge Flow to Split Vent 50 mL/min at 2 min
Thermal Aux 1 {MSD Transfer Line}
- Heater On
- Temperature Program On
320 °C for 0 min
- Run Time 10 min
Column #1
- HP-5MS 5% Phenyl Methyl Silox: 0001
- HP-5MS 5% Phenyl Methyl Silox
- 325 °C: 30 m x 250 µm x 0.25 µm
- In: Front SS Inlet He
- Out: Vacuum
- (Initial) 55 °C
- Pressure 0.54761 bar
- Flow 1 mL/min
- Average Velocity 36.534 cm/sec
- Holdup Time 1.3686 min
- Flow Program Off
- 1 mL/min for 0 min
- Run Time 10 min
