BioBrick

Favorite Tianjin 2012 iGEM Team Parts

1 Working BBa_K821001 Reporter RFP Reporter with o-RBS Qinfeng Wu, Bin Jia 1059 2 Working BBa_K821002 Coding Orthogonal 16S DNA sequence from rrnB Qinfeng Wu, Bin Jia 1894 3 Working BBa_K821012 Generator PBad(promoter)+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 3226

Tianjin 2012 iGEM Team Parts Sandbox

1 Working BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894 2 Working BBa_K821004 Regulatory PBad Promoter Jinlai Zhang, Dongchang Qin 1324 3 Working BBa_K821005 Reporter CmR label from pKD3 Qinfeng Wu, Bin Jia 1014 4 Working BBa_K821006 Project KanR label from pKD3 Jinlai Zhang, Dongchang Qin 1304 5 Working BBa_K821007 Composite PL(promoter)+orthogonal 16S RNA Qinfeng Wu, Bin Jia 1957 6 Working BBa_K821008 Composite PLtet+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 1956 7 Working BBa_K821009 RBS Orthogonal RBS 1 Qinfeng Wu, Bin Jia 6 8 Working BBa_K821010 Composite PLacI+Native RBS+RFP+Native RBS+GFP+Terminator Qinfeng Wu, Bin Jia 1833 9 Working BBa_K821003 Composite PLacI+Orthogonal RBS+RFP+Native RBS+GFP+Terminator Jinlai Zhang, Dongchang Qin 1827

Agarose gel electrophoresis

7.14 t7 rbs' crtE

7.31 16s' RFP-GFP AMP

8.03 pSB1C3 K3 Digestion

8.16 16s' mutate to remove EcoRI

8.17 16S' pSB1C3

8.17 Mutate the RBS of Amp using PYES2

8.17 Yeast Assembly using pSB3C5R & pSB3K3

8.18 pBad-16S' remove EcorI, PSB1C3

9.2 6I 6G Gel extraction and verification

9.2 6I,6G P16S Gel extraction and verification

9.2 P16S digestion

9.16 yeast cPCR 1

9.16 yeast cPCR 2

81 16s GFP & RFP