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Team:Bielefeld-Germany/Labjournal
From 2012.igem.org
Contents |
Week 1 (30.04.-06.05.12)
- Start of our WET LAB time.
- Generating new competent E.coli cells.
Week 2 (07.05.- 13.05.12)
- Doing the first steps to prepare a the Student Acadamy in cooperation with the CeBiTec. Finding suitable BioBricks to develop an coulourful and easy experiment. Trying to isolate the GFP and RFP containing plasmid and to transform an Plasmidmix to produce colored Agar-plates.
- Successful PCRs of laccase genes CopA from Xanthomonas campestris B100, CueO from E. coli BL21(DE3) and CotA from Bacillus pumilus ATCC7061 with genomic DNA as template. The primers were designed with T7 promotor-overhanging ends after prefix and HIS-Tag before suffix.
Week 3 (14.05.- 20.05.12)
- Cloning of CueO (E. coli), CopA (Xanthomonas campestris) and CotA (Bacillus pumilus) in pSB1C3-backbone.
Week 4 (21.05.- 27.05.12)
- Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.
Week 5 (28.05.- 03.06.12)
- Cloning of CotA (Bacillus halodurans), Tth (Thermus thermophilus) and ccdB in pSB1C3-backbone.
