Western Blot result from 8.sep.12

• Lane 1: Ladder
• Lane 2: 10(or 30) Lovtap Sample 1(100% lovtap)
• Lane 3: 10(or 30) Lovtap Sample 2(100% lovtap)
• Lane 4: 10(or 30) Lovtap Sample 3(50% lovtap+50% filler)
• Lane 5: 10(or 30) Lovtap Sample 4(50% lovtap+50% filler)
• Lane 6: 20(or 40) Lovtap Sample 1(100% lovtap)
• Lane 7: 20(or 40) Lovtap Sample 2(100% lovtap)
• Lane 8: 20(or 40) Lovtap Sample 3(50% lovtap+50% filler)
• Lane 9: 20(or 40) Lovtap Sample 4(50% lovtap+50% filler)
• Lane 10: 20(or 40) non-transfected CHO cell as a control + 5microG of VP16 only for the first membrane

Membrane was dirt by wrong secondary antibody and not washed well.

Cannot confirm the Lovtap-vp16 expression.

We found that there was membrane transferring problems also as you could see there are many white holes on the membrane.

We have to practice more on the membrane transfer.

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