Laboratory Notebook

23-07-2012 to 29-07-2012

SST send for sequencing

SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.

Cryo backup, miniprep and preparation to sequencing

We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector. These will be shipped of to be sequneced.

SST send for sequencing

The two sample from earlier were prepared for sequencing by diluting them to 70-78 ng/μl and then send. Afterwards we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing.