Notebook
- Our team pushed forward an experiment while making trial and error every day.
Creating parts of Tar methylation region
- Date:8/9
- Colony PCR
- →Reflection:We Took E.coli too many.You should take less E.coli.
- →Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)
- Date:8/11
- The purified DNA
- →Reflection:Band was less.
- PCR Product
- Date:8/13
- Confirmed of electrophoresis by PCR product and Ligation of the TA vector
- Date:8/14
- Transformation
- Date:8/25
- Miniprep
- Date:9/10
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- Date:9/11
- Primer design
- Date:9/12
- Miniprep,Ligation
- Date:9/13
- Electrophoresis,Refinement of DNA
- Date:9/14
- Colony PCR
- Date:9/15
- Ligation
- Date:9/16
- PCR,Ligation
- Date:9/17
- PCR,Ligation
- Date:9/19
- Ligation
- Date:9/20
- Enzyme inactivation,Ligation
- Date:9/22
- Transformation,Colony PCR,Electrophoresis,Miniprep
- Date:9/23 Colony PCR,Electrophoresis,Streak
- Date:9/25 Colony PCR
Creating parts of azurin
- Date:8/16
- Colony PCR
- Ligation
- Date:8/18
- Colony PCR
- Date:8/20
- DNA extraction and purification of P.aeruginosa
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