Team:Colombia/Notebook/Journal

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Chitinase

To determine which chitinase is going to be used, we screened chitinase from 3 different species since the chitinase of different Vibrio spp. was not suitable or the strain was found (Alivibrio fischeri ES114, Alivibrio fischeri M11):

- Arabidopsis thaliana

- Colletotrichum spp.

- Trichoderma spp.

For each one we obtained accesion numbers:

- Arabidopsis thaliana: [http://www.ncbi.nlm.nih.gov/nuccore/AY099810.2 AY099810.2]

- Colletotrichum spp. : [http://www.ncbi.nlm.nih.gov/nucest/GW342409.1 GW342409.1]

- Trichoderma spp.  : [http://www.ncbi.nlm.nih.gov/nucest/BM077089.2 BM077089.2]


Each one was tested using codon usage for bacteria (transl_table=11, [www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi NCBI link]) to determine if the chitinase could be used. All of the sequences were suitable for use.

We selected Colletotrichum spp. and Trichoderma spp. sequences to design primers.

Primers

- Colletotrichum spp. : - Trichoderma spp.  :

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