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NOTEBOOK - JUNE
- Redo amplification by using PCR, following by run gel of Backbone Vector A.
- Amplification by using PCR, following by run gel of Backbone Vector C. Incorrect banding shown that the PCR amplification was failed.
- Amplification by using PCR, following by run gel of Backbone Vector D. Incorrect banding shown that the PCR amplification was failed.
- Redo PCR amplification of Backbone Vector B, following by run gel. Correct banding shown and Backbone Vector B I is being successfully amplified.
- Redo PCR amplification of Backbone Vector D, following by run gel. Correct banding shown and Backbone Vector D I is being successfully amplified.
- Overlapping PCR of NpSRII-NpHtrII-Ectsr-backbone which the plasmid is responsible in repelling from blue light.
- Redo PCR amplification of Backbone Vector C, following by run gel. Correct banding shown and Backbone Vector B I is being successfully amplified.
- Overlapping PCR of NpSRII-NpHtrII-Ectar-backbone which the plasmid is responsible in attracting by blue light.
- Overlapping PCR of HsSRI-HsHtrI-Ectsr-backbone which the plasmid is responsible in repelling from orange light.
- Overlapping PCR of HsSRI-HsHtrI-Ectar-backbone which the plasmid should be responsible in attracting by orange light.
- Direct transformation of NpSRII-NpHtrII-Ectsr-backbone gene, follow by spread plate.
- Make plate with 0.5% soft agar with antibiotics- Ampiclin.
- Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
- Direct transformation of HsSRI-HsHtrI-Ectsr-backbone gene, follow by spread plate.
- Direct transformation of HsSRI-HsHtrI-Ectar-backbone gene, follow by spread plate.
- Pick clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.
- Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells. There is no clone on the plate. Direct transformation need to re-do.
- Pick clones from transformed HsSRI-HsHtrI-Ectsr-backbone cells.
- Pick clones from transformed HsSRI-HsHtrI-Ectar-backbone cells.
- Direct transformation of NpSRII-NpHtrII-Ectar-backbone gene, follow by spread plate.
- Pick clones from transformed NpSRII-NpHtrII-Ectar-backbone cells.
- Miniprep of picked clones from transformed NpSRII-NpHtrII-Ectsr-backbone cells.
- Plasmids containing NpSRII-NpHtrII-Ectsr-backbone gene were sent to sequencing.
- Sequencing results shown that the sequence of plasmids containing NpSRII-NpHtrII-Ectsr-backbone gene are incorrect.
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