We have turn engineering a gene into engineering a pathway, and we have lots of approaches to maintain the stability of the whole system. In terms of cells, it is difficult to satisfy what we need only by engineering a cell, because we require a mixture of different kind of cells to do more complex things. However, it is still very difficult for us to build such a mixed system, and the priority is how to balance suitable rate of different cell quorum. A suitable proportion is about correct result and max efficiency.
To accomplish this function, we found several proteins that inhibit E.coli replication initiation, and also built a quorum density inducible bistable switch to gain new balance.
This system is not only useful for acquiring suitable proportion of a mixed culture quorum, but also of great theoretical meaning and Application value as we proved.
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