Team:Korea U Seoul/Notebook/Jul

     Limitation: since input and output signal are identical, it is possible for input signal to diffuse into next logic gate.

     Solution: Degradation of input signal before diffusing to next logic gate is required.

     9 plasmids are needed for full adder.

      As DNA gets longer, DNA synthesis is expensive and even more when protein is attached to DNA

      Numerous of companies can make florescence DNA

      MIDLAND, for example, can make DNA segment; $2.6 per base pair and $325 for protein attachment

     Microbacterial “Damagochi” : using sigma factor, bacteria can ‘express’ their current condition: developed idea based on brainstorming session

    &nbspFor example       Sigma32 : heat shock (approximately 42degree)
      Sigma38 : starvation or stationary phase

     Professor is on seminar this week, so our meeting with professor is delayed.

     Further research and development of idea

     logic gate based on plasmid: . Hyunkee Kim, Kyeongwoo Jang, Sanghoon Han, Jihoon Jung

     logic gate based on DNA: Jeongmin Lee, Kyeongjin Kim

     back up idea: Jihyong Nam, Byeongnam Min, Haerim Song, Dohoon Kwon Kim

     Each team should submit one ppt files

      We had discussed with professor Choi, and he said that DNA logic gate is hard project. He added this project is not ingenious at al

      Thus, we had time to think about other topics in the meetings

     Jihoon Jung: Expiration date bacteria: when dairy food is no longer good, bacteria which are attached to the product as a form of sticker will emit florescence

     This idea is similar to bacteria timer. This idea is creative in a way that it also considers temperature and time. When temperature is up, growth of bacteria is stimulated and florescence will be synthesized faster and vice versa

     Further research and development of idea & new team allocation

     Wiki team: Byeongnam Min, Kyeongwoo Jang, Sanghoon Han

     Sponsor team: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song

     Human practice: Byeongnam Min

     Lab team: Hyunkee Kim, Kyeongwoo Jang, Sanghoon Han, Jihoon Jung

      Xanthomonas oryzae is pathogen which affects rice. In fact, Korea is the first country to sequence the genome of this pathogen due to its devastating effects on agriculture.

      This bacteria primary use small AvrXa21 protein as a communication chemical

      1. raxA, raxB, raxC, raxST are genes that synthesize proteins which are used for secrete AvrXa21

      2. raxH, raxR are used for sensing AvrXa21

      3. raxP, raxQ produce AvrXa21

      We will use raxH, and raxR which detect AvrXa21 for cloning. When it detects AvrXa21 it will synthesize florescence

      Once we are successful, we will replace bacteriocin instead of florescence.

      We need AvrXa21 to run some tests. Since it is difficult to colonize Xanthomonas oryzae, we are planning to synthesize AvrXa21 chemically. On the other hand it is difficult to put sulfur to the protein, so we need to research more about that

      Which promoter is responsible for binding with raxR output (TF) is unknown For cloning, EcoRI, XbaI, SpeI, PstI site should be excluded in genes, RaxRH has EcoRI, PstI restriction enzyme site, so we need mutant gene

     Synthetic biology presentation on February

     Review article submit: BT news

     CCP

     Highschool student iGEM collaboration

     KAIST Team collaboration

     Does it work without surfur attached toAvrXa21 : everyone

     promoter used for AvrXa21: everyone

     (optional) a way to selectively kill Xanthomonas

     A way to colonize Xanthomonas : Sanghoon Han

     Science camp presentation: Haerim Song

     Report for Sponsor: Jeongmin Lee, Kyeongjin Kim, Jihyong Nam, Haerim Song

     Wiki team: Byeongnam Min, Kyeongwoo Jang, Sanghoon Han