Team:Paris-Saclay/Project/Notebook
From 2012.igem.org
Revision as of 06:46, 26 September 2012 by YohannPetiot (Talk | contribs)
GEMOTE
![](http://www.igem-paris-saclay.u-psud.fr/wordpress/wp-content/uploads/2012/07/Aud.jpg)
![](http://www.ecole-adn.fr/uploads/2011/09/Probiotics-300x300.jpg)
GEMOTE
-
First WeekOpen or Close
-
Second WeekOpen or Close
-
Third WeekOpen or Close
-
Fourth WeekOpen or Close
-
Fifth WeekOpen or Close
-
Sixth WeekOpen or Close
-
Seventh WeekOpen or Close
-
Eighth WeekOpen or Close
-
Ninth WeekOpen or Close
-
Tenth WeekOpen or Close
6th august
- Sending of the B1 sample to sequencing with two pairs of primers
- K-274100 Forward and Reverse
- Plasmid pSB1A2 Forward and Reverse
7th august
- Liquid culture of B1 in order to prepare a glycerol stock
- Receipt of new primers
- 2 Forward
- 3 Reverse
- Plasmid Reverse
8th august
Amplification of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 by PCR with the new primers. Visualization by electrophoresis on a 2% Agarose gel for BBa_K115017 and a 0.8% Agarose gel for BBa_K274100 and the plasmid pSB1A2. We are expecting a band at 147 bp for BBa_K115017, 3408 bp for BBa_K274100 and 2079 for the plasmid.
9th august
New amplification by PCR of the plasmid pSB1A2, BBa_K274100 and BBa_K115017 as it has been done the day before.
Digestion by EcoRI of the plasmid that contains BBa_K274100.
-
Eleventh WeekOpen or Close
-
Thirteenth WeekOpen or Close
Follow us !