Team:Macquarie Australia/Protocols/RD

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Restriction Digest

We prepared Master Mixes containing to following volumes,

EcoR1 + Spe1 Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Spe12.5
Water75
Total100
Xba1 + Pst Master Mix
SubstrateVolume (µL)
10x Buffer20
Xba12.5
Pst2.5
Water75
Total100
EcoR1 + Pst Master Mix
SubstrateVolume (µL)
10x Buffer20
EcoR12.5
Pst2.5
Water75
Total100

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