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Team:Bielefeld-Germany/Labjournal/week22
From 2012.igem.org
Contents |
Week 22 (09/24 - 09/30/12)
Weekly Meeting
- First plan for the team t-shirt: a pacman eating estrogen.
- We now gather every day 9 o'clock in the morning to work on the wiki.
- Julia V is implementing the last design changes to the wiki.
Monday September 24th
- Team Cultivation & Purification:
Figure 1: Cultivation from 09/23 of E. coli KRX with <partinfo>BBa_K729006</partinfo> , E. coli KRX without plasmid (negative control) and with <partinfo>BBa_K863005</partinfo> (positive control). 60 mL in 250 mL flasks without baffles,LB-medium or autoinduction medium for <partinfo>BBa_K863005</partinfo> , 60 µg/mL chloramphenicol, 37 °C for 12 hours.
- For collaboration with UCL London: Made celldisruption via sonification and SDS-Page from lysates. The bands were analysed by MALDI-TOF.
Tuesday September 25th
Wednesday September 26th
Thursday September 27th
Friday September 28st
Saturday September 29nd
Sunday September 30rd
Sunday
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