Team:TU Darmstadt/Protocols/TRM-EDX

From 2012.igem.org

TEM-EDX-Measurements

Energy-dispersive X-ray spectroscopy (EDS or EDX) is an analytical technique to investigate the elemental composition and chemical characterization of a sample. Our sample is put into an electron microscope and radiated with an electron beam. Due to the radiation electrons from the inner shell of the atoms in the probe are driven out and create an electron hole. While electrons from the an outer shell fall down into this hole they radiate an energy spectra. This spectra is characteristic for every element and gives us the total composition of the radiated probe. We use EDX measurements to proof the functionality of our chosen tricarboxylat tripariate transporter. If the transporter is functional the composition of C and O atoms in the cytosol of our microorganisms must be different from probes without the transporter.