Team:LMU-Munich/Data/Inverter

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Team-LMU Photo9.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

Sporenfreunde

ONPG assay of the Inverter with lacZalpha

The Inverter is composed of the pBAD promoter (lacking the repressor binding sites) which produces the short RNA RyhB. RyhB binds to uof and masks the Shine Dalgarno sequence, so translationally repressing it. Uof is translationally fused to lacZalpha, and therefore is also translationally repressed. This is produced by an [http://partsregistry.org/Part:BBa_R0011 IPTG-inducible Promoter] which is in the ONPG-assay at all points induced with 1mM IPTG. The pBAD is titrated with Arabinose, and the higher induced, the higher the RyhB concentration and therefore the higher the lacZalpha translational repression. Above one can see the output of this construct, which produces less lacZalpha the more pBAD is induced.

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