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Team:NCTU Formosa/HumanPractice
From 2012.igem.org
Introduction
NCTU Formosa team did a lot of work on Human Practices, endeavoring to get people in other areas to know the great power of Synthetic Biology. We held a Bio-camp for high school students and did iGEM Cooperation throughout the country. We even tried to do Surveys from high school students, netizens around the world and professors from National Chiao Tung University and made L-shaped folders to voice to the world. And the most important one, we created a Facebook Page for those interested in Biosynthetic Biology who can now follow up our works.
Details
Bio-camp
Schedule for our Bio-camp
Day | Time | Activities | Host |
|---|---|---|---|
Two |
9:00~9:30 |
Introduction |
PENG, KAI-CHUN |
9:30~10:00 |
a. Basic biosynthetic biology |
Chung, Chia-Jung |
|
10:00~12:00 |
Experimental method and our iGEM topic introduction |
TSAI,LU-AN |
|
Four |
8:00~12:00 |
Bacteria colony number test |
PENG, AI-CHUN, |
Plasmid purification |
|||
Electrophoresis |
|||
Five |
10:00~12:15 |
Genomics research center visit |
Dr. Wu |
Six |
8:30~12:00 |
Q&A and group discussion |
TSAI,LU-AN |
Day 2
At the second day of the camp, we prepared a lecture for introducing the future development of synthetic biology; this day we were aimed to let the students know some basic knowledge as well as the competition methods of iGEM and how we did the experiments.
We did our best to introduce many contents in easy way. However, Mr. Tsai captured the students’ attention by his humorous speech, so that he turned the abstract and hard molecular biology concept in an easy way. Moreover, students also took their notes hard. At the end, they could totally get into the profound of the synthetic biology. Miss. Zhong introduced many interesting applications of synthetic biology and amazed the students. They also contributed some interesting ideas during the Q&A time.
We answer students’ questions anytime anywhere and helped them catch the main concepts of the synthetic biology.
Overall, we first introduced the development of the synthetic biology,then the origin, the competition and where the iGEM competition is held.
And we talked about some basic biobricks to yield our target proteins, and introduced the functions and properties of the enzymes that were usually used.
In order to let students have more complete structure of experiment, we design a cyclic illustration to represent the relations of each step, and introduced each experiments contents and necessities by simple words. To allow students clearly understand the concepts and methods of the synthetic biology, we edited some basic introduction and easy examples into the handouts. So they could understand the course easily as well as do exercises right away to test themselves whether they really understood what we had taught in the class. Because most of them were year 2 Senior high students and part of them were year 3 Senior high students, their background knowledge are different. As a result, we needed to allow year 2 Senior high students to understand but would not be too easy for year 3 Senior high students. And it was also a challenge for us to edit the handouts. Finally, we chose some examples like the regulator mechanism of the lactose, the fluorescence mark of the protein, etc. These were all questions we wanted to test themselves, and we hoped them could learn a lot from the courses.
Day 4
On the fourth day of biotech-camp, we prepared special arrangements. A half-day experimental course for students to experience the experimental techniques which our team used frequently: bacteria culture, plasmid extraction, electrophoresis, etc.
Then we explained the steps and principle of experiment by PowerPoint clearly to increase the familiarity of experimental operation, and prevent any accidents from happened. After the experiment began, we put our iGEM team members into each group so that they can avoid making mistakes during the experiment and to help the students in understanding the experimental technique.
The E.coli we prepared for them had been transplanted red fluorescent protein gene, and it is in beautiful red color at the beginning. But when we added the solution II which is need for taking the plasmid out of the bacteria, the color changed suddenly into bright yellow. What’s more, when we added the solution III, it changed form yellow into colorless. This significant color change phenomenon made the student surprised and to give rise to their curiosity on iGEM, finally, aroused their passion on the experiment.
Day 6
The fifth day we had a trip to visit the supreme academic organization in Taiwan. It is the Genomics Research Center, Academia Sinica. We invited two professors to join us, and hoped that they can answer the questions of the students. Genomics Research Center also generously provided us the opportunity to visit their lab and they explained how the equipment works in detail. And we also explored the cutting-edge technologies of genomics by their lecturer’s speech.
Day 6
In the last day of the camp, we held a competition to test the learning achievement of students. In order to test the knowledge that each group member learn in previous days. therefore, we designed some question on genetic engineering to make them think. And then we invited Mr. Tsai who is very energetic and humorous to host the competition. First, they should listen to the question and write the answer onto the whiteboard we had provided for each group. Second, they should show the answer to everyone. Then at last, we give scores according to their creativity and feasibility of their answer, just like a real iGEM competition, so that the students could personally experience the presence of competition. Due to the special charm of the host, the atmosphere is extremely tense and exciting. It is very difficult to decide who will win or who will lose between the groups. The points between each team were very close, so every students tried hard to recall the lessons they learned, hoping to win for the team. I believe the knowledge and experience they have learned in this six-day camp was precious. And they’ll never forget this memorable trip.
iGEM Cooperation
Hoping to be able to communicate and learn from each other, a cooperating platform, which contains NYMU-Taipei, NTU-Taida, NCTU_Formosa, was built. We discussed the popularization and application of the synthetic biology in this platform.
On the experimental aspect, just like a real and formal iGEM competition, each group presented their own topic in an extremely formal and extraordinary style, hoping that more information could be given to the audience in limited time; after each group finished their presentation, there would be a time for Q & A in which everyone could asked question in case they didn’t understand, they may solved their experimental problem for the other team either to exclude or find better experimental methods. On human practice aspect, on one hand, in order to promote the synthetic biology efficiently, the three teams think of ideas and shared the creative and effective ideas together hoping that we could broaden the scope of the human practice to allow more people to know synthetic biology as well as increase the visibility of iGEM; on the other hand, we could share things each team have been done to understand each other.
The most important things we have done on promoting synthetic biology to more school were that we spend much time on editing a synthetic biology guiding book in which there were many experimental protocols as well as simple illustrations which could help people know the purposes of experiments easily and that we also generously let everyone Download Our Guiding Book. Currently, NTU-Taida and NYMU-Taipei have already own our guiding book, and we welcome anyone who wants to know how to do experiment to read our guiding book. Finally, we deeply believed that people would get interested in molecular experiment more after reading our guiding book.
Popularization on Campus
We made a short film introducing iGEM , hoping that we can promote this interesting, brain-storming competition to everybody. We described iGEM as an once-in-a-lifetime experience, that anybody with a big heart & strong mind can participate in it. We play this film in the student diner at lunch time, so people can relax and see this film like they’re seeing a movie.
After watching the short film above, some students told us that they want to know more about iGEM. So we made another film, sharing our experience on our experiments, trial-and-error learning, joining the iGEM final competition in MIT, and other feelings that can't be express by words. We’re glad that many students from different field of studies told us that they’re willing to join iGEM 2013. We believe that they can surely contribute great and brilliant ideas to iGEM!
Popular Science Creation
Department of Biological Science and Technology and Department of Communication and Technology cooperate to make a Brief introduction to synthetic biology.
With the skill of CT and knowledge of DBT, we make a video with four simple question and following answer.
We hope this short interview can help general public understand the applications and techniques,as well as the influence of synthesis biology. Also we wish that this video can decrease irrational fear of Genetically modified creature, and build the positive concept.
This short interview will put on the popular science website – KEBOWEN, sharing with the internet to the whole Chinese area.
Surveys
(developing)
L-shaped Folders
We make colorful double-side-L-shaped folders, which are designed by De-Jung Tseng. We hope that those who received our folders can have a deep impression on NCTU iGEM team as well as our experimental topic --- E. coline, and our experimental purposes --- Solving the problem of energy crisis and air pollution, life would then be better by our project.
Our designed ideas are below:
On the front side shows a blackboard sketched with our ideas of producing biosynthetis isobutanol to make a better life. For the left part, we illustrate the pathway that indicates how cellulose is metabolized to isobutanol by constructing the enzymes, while for the right part shows two major method to increase the production rate of biosynthetic isobutanol , temperature control system and zinc finger utilization. The enzymes are indicated as the robots with their specific zinc finger shown in the triangle sign. The temperature control system is illustrated as the traffic light between IlvD and KivD.
The back side strictly shows the synthesis and application of isobutanol produced by our project , Eco.Fuel Ecoline. With the production line of Eco.Fuel , isobutanol would be easily accessible and widely used to replace gasoline that leads to air pollution.
