Transfections

Show the 30-50% efficiency.

Flowcytometry

Detecting LovTAP

Talk about shifting peak (and why absent in PBS one: dsRed is degraded by formaldehyde)

Characterizing LovTAP

Non-leakage DsRed, where art thou?

Part assembly

We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the [http://partsregistry.org/Main_Page Registry]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.

LovTAP-VP16: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]

LovTAP readout (RO): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]

Melanopsin: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]

NFAT response element: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003 BBa_K838003]